If no urine is visible in the catheter tubing:
cleans hands with alcohol hand rub, or washes with soap and water and dries with paper towels, dons a disposable plastic apron and non-sterile gloves prior to manipulating the catheter tubing.
;Applies non-traumatic clamp a few centimetres distal to the sampling port. Removes gloves and disposes appropriately.
;Cleans hands with alcohol hand rub, or washes with soap and water and dries with paper towels, following WHO guidelines.
;Dons non-sterile gloves.
;Wipes sampling port with 2% chlorhexidine in 70% isopropyl alcohol and allows drying for 30 seconds.

If using needle and syringe:
inserts needle into port at an angle of 45°, using a non-touch technique, and aspirates the required amount of urine, then withdraws needle.
If using needless system:
inserts sterile syringe firmly into centre of sampling port (according to manufacturer’s guidelines) using a non-touch technique, aspirates the required amount of urine, and removes syringe.
;Transfers an adequate volume of the urine specimen (approximately 10ml) into a sterile container immediately.
;Discards needle and syringe into sharps container (if relevant).
;Wipes sampling port with 2% chlorhexidine in 70% isopropyl alcohol and allows drying for 30 seconds.
;Unclamps catheter tubing (if relevant).
;Disposes of equipment including apron and gloves appropriately